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Initial Classification Form for Research Involving Recombinant or Synthetic Nucleic Acid Molecules
This information is required to determine if your research is exempt from IBC oversight based on Federal requirements (see HYPERLINK "https://osp.od.nih.gov/wp-content/uploads/NIH_Guidelines.pdf"NIH Guidelines for Research Involving Recombinant and Synthetic Nuclei Acid Molecules). If your research is exempt, you will receive notification from the IBC. Otherwise you will be required to comple t e a n a d d i t i o n a l f o r m f o r I B C r e v i e w . F o r m o r e i n f o r m a t i o n p l e a s e s e e I B C w e b s i t e : r e e d . e d u / i b c
S u b m i t t h e c o m p l e t e d f o r m t o i b c @ r e e d . e d u
P r i n c i p a l I n v e s t i g a t o r ( L a s t N a m e , F i r s t N a m e , D e g r e e ) E m a i l A d d r e s s T e l e p h o n e N u m b e r F O R M T E X T F O R M T E X T F O R M T E X T L a b l o c a t i o n ( b l d g . , r m ) ( o p t i o n a l ) L a b o r a t o r y C o n t a c t F O R M T E X T F O R M T E X T P r o j e c t T i t l e P r o j e c t S p o n s o r F O R M T E X T F O R M T E X T
P l e a s e c h e c k a l l t h a t a p p l y t o y o u r r e s e a r c h p r o ject and teaching lab activities:
FORMCHECKBOX This research involves transformation of standard laboratory strains of bacteria or yeast (e.g., Escherichia Coli K-12, Saccharomyces cerevisiae, Saccharomyces uvarum, or any asporogenic Bacillus subtilis or Bacillus licheniformis) with standard plasmids (see Appendix C of NIH Guidelines).
FORMCHECKBOX This research uses a host-vector system that is NOT Escherichia Coli K-12, Saccharomyces cerevisiae, Saccharomyces uvarum, or any asporogenic Bacillus s u b t i l i s o r B a c i l l u s l i c h e n i f o r m i s . ( s e e A p p e n d i x C o f N I H G u i d e l i n e s ) . I f c h e c k e d , p l e a s e l i s t s y s t e m ( s ) u s e d : F O R M T E X T
F O R M C H E C K B O X T h i s r e s e a r c h i n v o l v e s t r a n s f e c t i o n o f t i s s u e c u l t u r e c e l l s w i t h n o n - v i r a l v e c t o r s ( e . g . , p l a s m i d s ) .
FORMCHECKBOX This research uses viral vectors or any vector containing >50% of the genome of a eukaryotic virus.
FORMCHECKBOX This research involves the use of synthetic nucleic acid molecules in cells, organisms or viruses. If checked, please check all that apply below:
FORMCHECKBOX The synthetic nucleic acid molecule can replicate or generate nucleic acids that can replicate.
FORMCHECKBOX The synthetic nucleic acid molecule can integrate into host DNA.
FORMCHECKBOX This research involves transgenic/knockout rodents.
FORMCHECKBOX This research involves the creation of other transgenic animals (e.g., flies, fish), including the generation of new strains by breeding two different strains.
FORMCHECKBOX This research involves the use of recombinant plasmids/vectors/viruses, including recombinantly modified cells/organisms, in animals (other than the creation of transgenic animals as noted above).
FORMCHECKBOX This research involves the deliberate transfer of a drug resistance trait to microorganisms that are not known to acquire the trait naturally, and acquisition could compromise the use of a drug used to control disease in humans, veterinary medicine, or agriculture (see Section III-A-1-a of NIH Guidelines).
FORMCHECKBOX This research involves the deliberate formation of recombinant or synthetic nucleic acid molecules containing genes for the biosynthesis of toxin molecules lethal for vertebrates at a LD50 of less than 100 ng/kg body weight (see Appendix F of NIH Guidelines).
FORMCHECKBOX This research involves DNA from or synthetic nucleic acid molecules encoding a significant human pathogen (defined as risk group 3 or 4) or cells known to be infected with these agents (see Appendix B of the NIH Guidelines). If in doubt, list proposed agent: FORMTEXT
F O R M C H E C K B O X T h i s r e s e a r c h i n v o l v e s m o r e t h a n 1 0 l i t e r s o f c u l t u r e i n a s i n g l e c o n t a i n e r .
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I agree that as principal investigator it is my responsibility to make certain that prior to engaging in research involving known or potential pathogens, all laboratory and support personnel are properly trained in the practices and techniques required to ensure safety, and to supervise the safety performance of those involved ensuring that the required safety practices and techniques are employed.
I agree to send a revised project description to the Institutional Biosafety Committee if changes are made to portions of this project involving recombinant or synthetic nucleic acids and will not begin work prior to IBC approval.
FORMTEXT P r i n c i p a l I n v e s t i g a t o r S i g n a t u r e D a t e
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